|
|
Useful Links
-
Winpcap
-
Windows Packet Capture library. This is required by tracetcp in order to function.
-
tcptraceroute
-
A traceroute program that uses TCP packets for Linux/Unix written by Michael C. Toren. This program (and the fact I couldn't find a Windows equivalent) was the inspiration for tracetcp.
-
Ethereal
-
A fantastic network protocol analyzer, that has saved my bacon numerous times. Anyone writing or supporting networked software should use this program.
-
Winsock FAQ
-
Very useful programmers information about Winsock programming.
-
Microsoft's Raw TCP/IP sockets Documention
-
Microsoft's rather short documentation about Raw sockets under Windows (not especially useful any more....).
-
IANA's list of port numbers
-
A handy list of the Well Known and Registered port numbers.
|


| 1 |
UNKNOWN_1991 |
Constitutive and basal secretion from the endocrine cell line, AtT-20 |
None |
1991 |
1991-03-01 |
NONCOMM |
N |
PMC2288887 |
1847928.0 |
|
tqgsavnr |
0.981263 |
UNKNOWN_1995, UNKNOWN_1996 |
Tooze_1990, UNKNOWN_1994, UNKNOWN_1989 |
| 2 |
Tooze_1990 |
Cell-free protein sorting to the regulated and constitutive secretory pathways |
Tooze, Sharon A.; Huttner, Wieland B. |
1990 |
1990-03-09 |
PMC |
N |
PMC7125605 |
2138058.0 |
10.1016/0092-8674(90)90097-x |
fj7sf345 |
0.900436 |
UNKNOWN_1995, Jonker_2018, Wang_2013, UNKNOWN_1992 |
UNKNOWN_1992, Stalder_2020 |
| 3 |
Klumperman_1998 |
Mannose 6–Phosphate Receptors Are Sorted from Immature Secretory Granules via Adaptor Protein AP-1, Clathrin, and Syntaxin 6–positive Vesicles |
Klumperman, Judith; Kuliawat, Regina; Griffith, Janice M.; Geuze, Hans J.; Arvan, Peter |
1998 |
1998-04-20 |
NONCOMM |
N |
PMC2148452 |
9548715.0 |
|
fa4ngoru |
0.855513 |
UNKNOWN_1995, Jonker_2018, Wang_2013 |
|
| 4 |
UNKNOWN_1994 |
Distinct molecular mechanisms for protein sorting within immature secretory granules of pancreatic beta-cells |
None |
1994 |
1994-07-01 |
NONCOMM |
N |
PMC2120086 |
8027188.0 |
|
o4pofqod |
0.835309 |
UNKNOWN_1995, Jonker_2018, Wang_2013 |
Klumperman_1998, UNKNOWN_1991 |
| 5 |
UNKNOWN_1991 |
Milieu-induced, selective aggregation of regulated secretory proteins in the trans-Golgi network |
None |
1991 |
1991-12-02 |
NONCOMM |
N |
PMC2289203 |
1757460.0 |
|
syyi2ysq |
0.732915 |
UNKNOWN_1995, Jonker_2018, Wang_2013, Tooze_1990 |
UNKNOWN_1995 |
| 6 |
Glombik_1999 |
The disulfide-bonded loop of chromogranin B mediates membrane binding and directs sorting from the trans-Golgi network to secretory granules. |
Glombik, M M; Krömer, A; Salm, T; Huttner, W B; Gerdes, H H |
1999 |
1999-02-15 |
PMC |
N |
PMC1171197 |
10022847.0 |
10.1093/emboj/18.4.1059 |
di3iae0s |
0.727381 |
Wang_2013, UNKNOWN_1995, UNKNOWN_1993, UNKNOWN_1987 |
UNKNOWN_1995 |
| 7 |
Tooze_1998 |
Biogenesis of secretory granules in the trans-Golgi network of neuroendocrine and endocrine cells |
Tooze, Sharon A |
1998 |
1998-08-14 |
PMC |
N |
PMC7126647 |
9714820.0 |
10.1016/s0167-4889(98)00059-7 |
w4zvfkyl |
0.682926 |
UNKNOWN_1995, Jonker_2018, Saraste_1992 |
Tooze_1990, UNKNOWN_1994, Stalder_2020 |
| 8 |
UNKNOWN_1987 |
Sorting of progeny coronavirus from condensed secretory proteins at the exit from the trans-Golgi network of AtT20 cells |
None |
1987 |
1987-09-01 |
NONCOMM |
N |
PMC2114808 |
2821011.0 |
|
hduv0ur4 |
0.652841 |
UNKNOWN_1995, Jonker_2018 |
UNKNOWN_1995 |
| 9 |
UNKNOWN_1992 |
Intermediates in the constitutive and regulated secretory pathways released in vitro from semi-intact cells |
None |
1992 |
1992-05-01 |
NONCOMM |
N |
PMC2289447 |
1572894.0 |
|
j3vo4zkj |
0.591523 |
UNKNOWN_1995, Jonker_2018, Tooze_1990, Wang_2013 |
Tooze_1990, UNKNOWN_1989, UNKNOWN_1991 |
| 10 |
Chanat_1991 |
What the granins tell us about the formation of secretory granules in neuroendocrine cells |
Chanat, Eric; Pimplikar, Sanjay W.; Stinchcombe, Jane C.; Huttner, Wieland B. |
1991 |
1991-01-01 |
PMC |
N |
PMC7090494 |
1726891.0 |
10.1007/bf02989882 |
mc93mope |
0.570214 |
UNKNOWN_1995, Jonker_2018, Hobman_1993, Saraste_1992 |
UNKNOWN_1994, Stalder_2020 |
| 11 |
UNKNOWN_1990 |
Sorting within the regulated secretory pathway occurs in the trans- Golgi network |
None |
1990 |
1990-01-01 |
NONCOMM |
N |
PMC2115992 |
2295680.0 |
|
ht3ry9i0 |
0.532603 |
UNKNOWN_1995, Jonker_2018, Wang_2013 |
Stalder_2020 |
| 12 |
Tooze_1991 |
Secretory granule formation: The morphologist’s view |
Tooze, John |
1991 |
1991-01-01 |
PMC |
N |
PMC7090665 |
1726882.0 |
10.1007/bf02989885 |
uzzoji4z |
0.519557 |
|
Stalder_2020, Chanat_1991 |
| 13 |
Ozber_2019 |
The role of post-Golgi transport pathways and sorting motifs in the plasmodesmal targeting of the movement protein (MP) of Ourmia melon virus (OuMV) |
Natali Ozber; Paolo Margaria; Charles T. Anderson; Massimo Turina; Cristina Rosa |
2019 |
2019-08-11 |
BioRxiv |
N |
|
|
10.1101/724716 |
bvcahbbi |
0.501394 |
UNKNOWN_1995, Jonker_2018 |
UNKNOWN_1996, UNKNOWN_1995 |
| 14 |
Cool_1994 |
Identification of a sorting signal for the regulated secretory pathway at the N-terminus of pro-opiomelanocortin |
Cool, D.R.; Peng Loh, Y. |
1994 |
1994-12-31 |
PMC |
N |
PMC7131109 |
7819333.0 |
10.1016/0300-9084(94)90156-2 |
og3hdnl2 |
0.497190 |
UNKNOWN_1993, UNKNOWN_1993, UNKNOWN_1987 |
|
| 15 |
Bowman_2010 |
Biogenesis of Dense-Core Secretory Granules |
Bowman, Grant R.; Cowan, Andrew T.; Turkewitz, Aaron P. |
2010 |
2010-05-27 |
PMC |
N |
PMC7122546 |
|
10.1007/978-0-387-93877-6_10 |
h5ttoo0z |
0.458905 |
UNKNOWN_1995, Jonker_2018, Hobman_1993 |
Stalder_2020, UNKNOWN_1995 |
| 16 |
Sridhar_2012 |
The lipid kinase PI4KIIIβ preserves lysosomal identity |
Sridhar, Sunandini; Patel, Bindi; Aphkhazava, David; Macian, Fernando; Santambrogio, Laura; Shields, Dennis; Cuervo, Ana Maria |
2012 |
2012-12-21 |
PMC |
N |
PMC3567500 |
23258225.0 |
10.1038/emboj.2012.341 |
dk1dl9bz |
0.457683 |
Jonker_2018, UNKNOWN_1995, Cosson_2013 |
Fu_J_2000, UNKNOWN_1994 |
| 17 |
Mironov_2008 |
Origins of the regulated secretory pathway |
Mironov, Alexander A.; Arvan, Peter |
2008 |
2008-01-01 |
PMC |
N |
PMC7121582 |
|
10.1007/978-3-211-76310-0_30 |
v8enwfhu |
0.436404 |
UNKNOWN_1995, Wang_2013, Jonker_2018, UNKNOWN_1993 |
Stalder_2020, Fu_J_2000, Tooze_1990 |


This is a multi-part message in MIME format.
=09
Dear Michelle,
You have an upcoming loan payment on 11/23/2015 for $451.63 for your Pro=
sper loan. No action is required on your part: the payment will be autom=
atically deducted from your primary bank account on file on 11/23/2015.
Automatic payment date: 11/23/2015
Payment amount: $451.63
Bank account: WELLS FARGO BANK NA, ****3812
Please make sure that your bank account has sufficient funds to cover=
your payment. If insufficient funds exist, you may be charged a failed=
payment fee by Prosper and an insufficient funds fee by your bank.
Since the payment transaction is initiated on the business day prior to=
the payment due date, any changes to your primary bank account must be=
completed two (2) business days prior to the payment due date.
Thank you for being a part of the Prosper community.
Regards,
Prosper
Notice to Servicemembers
Servicemembers on "active duty" or "active service" may be entitled to=
certain legal protections and debt relief pursuant to the Servicemember=
s Civil Relief Act (SCRA). For questions about the SCRA, servicemembers=
should contact their unit's Judge Advocate, or their installation's Leg=
al Assistance Officer. A military legal assistance office locator for=
all branches of the Armed Forces is available at http://legalassistance=
.law.af.mil/content/locator.php.
Dear Michelle,
You have an upcoming loan payment on 11/23/2015 for $451.63=
for your Prosper loan. No action is required on your part: the payment will be automatically deducted from your primary bank=
account on file on 11/23/2015.
Automatic payment date: 11/23/2015 Payment amount: $451.63 Bank account: WELLS FARGO BANK NA, ****3812
Please make sure that your bank account has sufficient funds=
to cover your payment. If insufficient funds exist, you may be charged=
a failed payment fee by Prosper and an insufficient funds fee by your=
bank.
Since the payment transaction is initiated on the business=
day prior to the payment due date, any changes to your primary bank acc=
ount must be completed two (2) business days prior to the payment due=
date.
Thank you for being a part of the Prosper community.
Regards,
Prosper
Notice to Servicemembers
Servicemembers on "active duty" or "active service" may be entitled to=
certain legal protections and debt relief pursuant to the Servicemember=
s Civil Relief Act (SCRA). For questions about the SCRA, servicemembers=
should contact their unit's Judge Advocate, or their installation's Leg=
al Assistance Officer. A military legal assistance office locator for=
all branches of the Armed Forces is available at htt=
p://legalassistance.law.af.mil/content/locator.php.


Related JIRA Ticket: SOHO-7051 Examples of SVG icons aligned with a label. See the source code of this test for more details.


| Candidate Name | Office | Party |
|---|---|---|
| Kingsley Moghalu | Presidential | Accord |
| Oby Ezekwesili | Presidential | Action Alliance |
| General M. Buhari | Presidential | All Progressive Congress |
| Omoyele Sowore | Presidential | Action Peoples' Party |
| Alhaji Atiku Abubakar | Presidential | Peoples Democratice Party |
| Uze Obakaliki | Governorship | Accord |
| Umar Jimoh | Governorship | Action Alliance |
| Babajide Sanwo-olu | Governorship | All Progressive Congress |
| Olayanju Sarumi | Governorship | Action Peoples' Party |
| Jimi Agbaje | Governorship | Peoples Democratice Party |


Money Play. How should Red play 54?
pips Red=140 White=123
(CorrectAnswer= 0/0, ErrorScore= 0)
Red needs to take advantage of White’s two home board blots.
We can discard the dreadful 13/9, 13/8, taking all the pressure off White. 9/5, 6/1* is a candidate, unstacking the heavy 6-pt and putting White on the bar.
However, the best play is 9/4, 8/4, strengthening the home board. The two Red blots are in little danger as White will need a perfect number to elect to hit.
Over the board 20/16, 20/15 was played but running while behind is rarely right and here it is worse than a double blunder.
Following Kent Goulding’s maxim: “put them where you want them” is often a good guide and here it is spot on. 9/4, 8/4 it is.
We can discard the dreadful 13/9, 13/8, taking all the pressure off White. 9/5, 6/1* is a candidate, unstacking the heavy 6-pt and putting White on the bar.
However, the best play is 9/4, 8/4, strengthening the home board. The two Red blots are in little danger as White will need a perfect number to elect to hit.
Over the board 20/16, 20/15 was played but running while behind is rarely right and here it is worse than a double blunder.
Following Kent Goulding’s maxim: “put them where you want them” is often a good guide and here it is spot on. 9/4, 8/4 it is.
1. Cubeful 2-ply 13/9 6/1* Eq.: +0.109 0.525 0.136 0.005 - 0.475 0.095 0.002 2. Cubeful 2-ply 9/4 8/4 Eq.: +0.100 (-0.009) 0.518 0.133 0.004 - 0.482 0.086 0.001 3. Cubeful 2-ply 20/16 6/1* Eq.: +0.099 (-0.010) 0.541 0.118 0.004 - 0.459 0.131 0.003 4. Cubeful 2-ply 6/2 6/1* Eq.: +0.095 (-0.014) 0.526 0.147 0.005 - 0.474 0.124 0.003 5. Cubeful 2-ply 9/5 6/1* Eq.: +0.088 (-0.021) 0.520 0.143 0.005 - 0.480 0.107 0.002


*Note: if you link a file with coordinates in World Geodetic System (lon/lat), please set in the "ADVANCED OPTIONS" the SRS to EPSG:4326


This page is using CanJS
CanJS devtools are integrated with the normal Chrome Developer tools
Open Developer Tools to find
- The Observable Properties Editor in the sidebar of the Elements panel
- The Bindings Graph in the sidebar of the Elements panel
- The Queues Stack in the sidebar of the Sources panel
For more info, see the
Debugging Guide.


About Me
I started to be curious about code in about 2018. And a teacher of mine gave me C++ Primer Plus as a gift. Since then, I started looking for the technologies I like.
I the summer of 2018, I even created my first blog without any site generators, all by my hands. Without CSS and JavaScript, I put all the stylesheets in attribute style
and all the buttons are powered by links.
I joined GitHub in 2019 with my websites.
By trying, learning and practicing JavaScript, CSS and more, a year was past.
In 2020, I became more confident in my skills. I published two packages, videoplayer.js and tooltiplery.js to npm in spring, 2020. They are no longer supported.
In summer, 2020, I created my new blog with Hexo, which you are currently visiting. Also, I made a brand new theme for my new blog - Theme Cupertino. Plus, I started contributing some open source projects.
I published a browser extension called GitHub Dark Mode to implementdark mode for GitHub.com.
In autumn 2020, GitHub officially implemented their dark mode, so GitHub Dark Mode was archived. For some enhanced visual features on GitHub.com, I published GitHub Enhancing. It adds acrylic and some animations to GitHub.com, which makes it more interactive.
In summer 2021, RSA CLI turned stable, and RSA GUI was in beta test.
The summer of 2022, I bought the domain mrwillcom.com
, so from then on, all my websites are migrated to it.
About This Blog
This blog is based on Hexo, automatically built by GitHub Actions. You can visit it from blog.mrwillcom.com (powered by Vercel) or mrwillcom.github.io (powered by GitHub Pages).
This blog also has a mirror on xLog, xlog.mrwillcom.com, part of my posts are mirrored there.
This theme is called Cupertino, it’s open source and you can get it here.
About this Post
This post is written by Mr. Will, licensed under CC BY-NC 4.0.


I'm Robert G. Raleigh — an agile IT project manager & frontend developer working in Portland, OR with 10+ years of combined project management, team leadership roles, and technology consulting experience since 2004.
I'll contract directly with your digital agency, or forward-thinking small-to-mid-size business to help your team plan, iterate, development, launch, and maintain your ongoing or upcoming small and large-scale technology projects.
Throughout my career, I've launched more than 100+ enterprise apps, mobile apps, custom CMS websites, e-commerce stores, and static sites, managing a client roster of 5 – 7 projects, with budgets ranging from $5,000 – $250,000.
I hold a Bachelor's Degree in English, from Portland State University, with a concentration in professional writing. I've also completed additional Management/Supervisory Development training at Portland Community College in project management and leadership.
Outside of work, I’m passionate about songwriting,slide blues guitar, and intergenerational learning. Most Sundays, I can be found in the yellow or blue room at Powell’s Books, looking for the next great mystery or short story writer.
More About RobertWe work in small teams using data to make creative solutions for our clients. Our process is simple, we listen before we create then we measure and repeat. We refine because we’re never satisfied. We test, hone, and customize our process to better suit your specific goals, and we work to redefine the digital experience in your industry.
We work in small teams using data to make creative solutions for our clients. Our process is simple, we listen before we create then we measure and repeat. We refine because we’re never satisfied. We test, hone, and customize our process to better suit your specific goals, and we work to redefine the digital experience in your industry.
Work with RobertHighly cost-effective, secure websites built using JAMStack using the leading and highly optimized static hosting, including Netlify, Firebase, like Jekyll, Netlify CMS,
View PackagesHighly cost-effective, secure websites built using JAMStack using the leading and highly optimized static hosting, including Netlify, Firebase, like Jekyll, Netlify CMS,
View PackagesHighly cost-effective, secure websites built using JAMStack using the leading and highly optimized static hosting, including Netlify, Firebase, like Jekyll, Netlify CMS,
View PackagesHighly cost-effective, secure websites built using JAMStack using the leading and highly optimized static hosting, including Netlify, Firebase, like Jekyll, Netlify CMS,
View PackagesI provide custom, easily managed and maintained CMS websites built on the industry-leading Drupal, Squarespace, Wordpress, and Wix platforms.
View PackagesEvaluating your technology options is difficult—especially with the pace technolgy changes. I'll help you to review your existing technology solutions, and plot a better course forward.
I provide custom, easily managed and maintained CMS websites built on the industry-leading Drupal, Squarespace, Wordpress, and Wix platforms.
View PackagesLet's grab a fresh cup of coffee, and discuss your new project or opportunity.
Let's talk, and you can get me up to speed on the problem you need solved.
Fill me in with some more project details and the challenges you're facing.
We've grabbed the following image from the gravatar image associated with your registered e-mail address.
Is it okay to use this photo?


Phase 2.1: Interior Extras
Once I'm done with the main phase 2 interior work I'll put these finishing touches
on the interior.
Replacement front door panels
The existing door trim panels are either origonal, or very close to it.
They're a mess. Sun baked, disintegrating, and ugly. I'm not sure
exactly what I'll replace them with, but probably panels from a wrecking
yard from a much more recent truck.
Dome light(s)
There are no lights in the truck right now, which makes finding stuff at
night a pain. I've got a flashlight in the center console, but that's
not a workable solution. Ideally I want to come up with something that
works with the top off as well, so I'll either integrate it into the
roll cage, or the kick panels.
Late model rear-view mirror with temp/compas
One thing I love about more modern cars is the exterior temp guage.
I head to Tahoe, and knowing when the road is freezing is a big win.
I'm going to install a temp/compas rear view mirror in the truck as it
wont require any funky hacks in the dash and should be a nice clean way
to get a temp guage in the truck.
re-appolster rear seat to match front seats
The folding rear seat I've installed is an old crappy blue cloth one.
I want to re-appolster it with a matching fabric to the front seat.
Laptop and diag software
Once of the great things about the 94-95 Chevy LT1 engine is the PCM
engine control compter can be hacked/controlled by consumers fairly
easily. There are multiple near-free software apps that will allow
you to get real-time data, as well as modify various aspects of the
computers behavior. Given that I'm a software guy myself, this is a
big win. I'm looking into getting a lower-end laptop and semi-perminently
installing it in the truck. I'll then be able to do real-time diagnostics
if anything should happen, see how things are going, amuse paseengers
by telling them the advance, temps, you name it. I'll also be able to
use the laptop for other uses, possibly MP3 playing, etc.
Back to my automotive page To my top level home page


Man shoots brother after mistaking him for deer, deputies say
by Theresa Seiger, Cox Media Group National Content DeskIONIA COUNTY, Mich. - A Michigan man shot his brother over the weekend after mistaking him for a deer while they were hunting, according to officials with the Ionia County Sheriff's Office.
Deputies said the 28-year-old victim, who lives in Lowell, was severely injured. He was taken to Spectrum Health Hospitals Butterworth Hospital in Grand Rapids, where he was listed Saturday in serious but stable condition.
The brothers told deputies they had gotten separated while searching for a deer in dense corn around 5:30 p.m. Saturday.
"The shooter in this case was some distance away and believed he saw the deer moving and heard the deer making noise," deputies said in a news release. The victim's 29-year-old brother, who lives in Saranac, opened fire and struck the 28-year-old with a shot from his muzzle-loading rifle, according to authorities.
Deputies said the 29-year-old called 911 for help immediately after he shot his brother. Deputies were investigating the case to determine the exact cause of the shooting. Officials said Saturday that all people involved in the incident were fully cooperating with investigators.


Dota Inventory is intended as a central resource for cosmetic items in Dota 2. The main idea behind this site is to allow you to find items that you like, share them with your friends and make it easier to find others who own the item in order to possibly initiate a trade.
A player's steamid is a unique identifier used by Steam that identifies the Steam account. A Community URL is a web address which links to a user's profile page. You can view your Community URL but will need to sign in through Steam.
A user's inventory is updated at most every 24 hours. However, a user's inventory will not be updated unless it is requested in some way. This prevents pointless updates and resource costs on the server.
Yes. Adding an item to your favourites will mean that you will be alerted when someone on your friend's list acquires this item. Note: you must check back here to find out.
Your watch list will (not yet implemented) allow you to save users (not necessarily friends) to a list that will let you know when they acquire new items. In particular, you will be alerted to when users on your watch list find items on your favourites list.
Not yet. This will definitely be implemented in the future but it may be a premium feature.
You can post a trade for an item if you own it. Users including yourself can then search for other items that other people are looking to trade.
No. There's a low possibility that a site like this for TF2 will be made in the future.


Cynthia Yim
About
Experience
Education
Skills
Interests
Profolio
Contact
Cynthia
Yim
Adelaide, SA 5000 ·<PHONE_NUMBER> ·
<EMAIL_ADDRESS>My LinkedIn
My Github
Porfolio
Horiseon
A Company Introduction Website
Work Day Scheduler
A simple calendar app for scheduling your work day.
Doggo App
An app that uses several APIs calls to introduce dog breeds (Group project).
-->


| These options correct your log K values (which must be valid for I=0) for the ionic strength. |
| The activities of all species are divided by the activity-term calculated according to one of the offered approximations, and new log K are calculated accordingly. |
| Mixed constants are used according to IUPAC convention. Therefore the ion product of water is defined as K={H+}[OH-]. |
| Conditions: | - | All log K values in the matrix must be valid for I=0. |
| - | All charges of species and components must appear in their names in the matrix as '+' or '-' characters, eg. "HCO3-", "Ca++", "PO4---" |
| Debye-Hückel-approximation: | for I<0.005M |
| Güntelberg-approximation: | for I<0.1M, useful in solutions of several electrolytes. |
| Davies-approximation: | for I<0.5M (const=0.2) |


PAR: Place And Route Diamond (64-bit) <IP_ADDRESS>.2. Copyright (c) 1991-1994 by NeoCAD Inc. All rights reserved. Copyright (c) 1995 AT&T Corp. All rights reserved. Copyright (c) 1995-2001 Lucent Technologies Inc. All rights reserved. Copyright (c) 2001 Agere Systems All rights reserved. Copyright (c) 2002-2017 Lattice Semiconductor Corporation, All rights reserved. Fri Mar 10 23:28:41 2017 C:/lscc/diamond/3.9_x64/ispfpga\bin\nt64\par -f SevenSegClock_SevenSegClock.p2t SevenSegClock_SevenSegClock_map.ncd SevenSegClock_SevenSegClock.dir SevenSegClock_SevenSegClock.prf -gui Preference file: SevenSegClock_SevenSegClock.prf. Cost Table Summary Level/ Number Worst Timing Worst Timing Run NCD Cost [ncd] Unrouted Slack Score Slack(hold) Score(hold) Time Status ---------- -------- ----- ------ ----------- ----------- ---- ------ 5_1 * 0 277.633 0 0.322 0 21 Complete * : Design saved. Total (real) run time for 1-seed: 21 secs par done! Lattice Place and Route Report for Design "SevenSegClock_SevenSegClock_map.ncd" Fri Mar 10 23:28:41 2017 Best Par Run PAR: Place And Route Diamond (64-bit) <IP_ADDRESS>.2. Command Line: par -w -l 5 -i 6 -t 1 -c 0 -e 0 -gui -exp parUseNBR=1:parCDP=0:parCDR=0:parPathBased=OFF SevenSegClock_SevenSegClock_map.ncd SevenSegClock_SevenSegClock.dir/5_1.ncd SevenSegClock_SevenSegClock.prf Preference file: SevenSegClock_SevenSegClock.prf. Placement level-cost: 5-1. Routing Iterations: 6 Loading design for application par from file SevenSegClock_SevenSegClock_map.ncd. Design name: AutoShift NCD version: 3.3 Vendor: LATTICE Device: LCMXO2-640UHC Package: TQFP144 Performance: 5 Loading device for application par from file 'xo2c1200.nph' in environment: C:/lscc/diamond/3.9_x64/ispfpga. Package Status: Final Version 1.42. Performance Hardware Data Status: Final Version 34.4. License checked out. Ignore Preference Error(s): True Device utilization summary: PIO (prelim) 89+4(JTAG)/108 86% used 89+4(JTAG)/108 86% bonded IOLOGIC 85/108 78% used SLICE 111/320 34% used OSC 1/1 100% used Number of Signals: 421 Number of Connections: 911 Pin Constraint Summary: 25 out of 89 pins locked (28% locked). The following 3 signals are selected to use the primary clock routing resources: osc_clk_c_c (driver: OSCH_inst, clk load #: 100) clk_c (driver: clk, clk load #: 48) latch_c (driver: latch, clk load #: 42) WARNING - par: Signal "clk_c" is selected to use Primary clock resources. However, its driver comp "clk" is located at "67", which is not a dedicated pin for connecting to Primary clock resources. General routing has to be used to route this signal, and it might suffer from excessive delay or skew. WARNING - par: Signal "latch_c" is selected to use Primary clock resources. However, its driver comp "latch" is located at "65", which is not a dedicated pin for connecting to Primary clock resources. General routing has to be used to route this signal, and it might suffer from excessive delay or skew.  However, its driver comp "pwm" is located at "50", which is not a dedicated pin for connecting to Secondary clock resources. General routing has to be used to route this signal, and it might suffer from excessive delay or skew. No signal is selected as Global Set/Reset. . Starting Placer Phase 0. ........... Finished Placer Phase 0. REAL time: 6 secs Starting Placer Phase 1. .................... Placer score = 61769. Finished Placer Phase 1. REAL time: 18 secs Starting Placer Phase 2. . Placer score = 61565 Finished Placer Phase 2. REAL time: 18 secs Clock Report Global Clock Resources: CLK_PIN : 0 out of 8 (0%) General PIO: 3 out of 108 (2%) PLL : 0 out of 1 (0%) DCM : 0 out of 2 (0%) DCC : 0 out of 8 (0%) Quadrants All (TL, TR, BL, BR) - Global Clocks: PRIMARY "osc_clk_c_c" from OSC on comp "OSCH_inst" on site "OSC", clk load = 100 PRIMARY "clk_c" from comp "clk" on PIO site "67 (PB18D)", clk load = 48 PRIMARY "latch_c" from comp "latch" on PIO site "65 (PB18C)", clk load = 42 SECONDARY "heartbeat_c" from Q1 on comp "SLICE_0" on site "R7C12C", clk load = 0, ce load = 0, sr load = 84 SECONDARY "pwm_c" from comp "pwm" on PIO site "50 (PB9B)", clk load = 6, ce load = 0, sr load = 0 PRIMARY : 3 out of 8 (37%) SECONDARY: 2 out of 8 (25%) Edge Clocks: No edge clock selected. I/O Usage Summary (final): 89 + 4(JTAG) out of 108 (86.1%) PIO sites used. 89 + 4(JTAG) out of 108 (86.1%) bonded PIO sites used. Number of PIO comps: 89; differential: 0. Number of Vref pins used: 0. I/O Bank Usage Summary: +----------+----------------+------------+-----------+ | I/O Bank | Usage | Bank Vccio | Bank Vref | +----------+----------------+------------+-----------+ | 0 | 24 / 28 ( 85%) | 3.3V | - | | 1 | 26 / 26 (100%) | 3.3V | - | | 2 | 24 / 28 ( 85%) | 3.3V | - | | 3 | 15 / 26 ( 57%) | 3.3V | - | +----------+----------------+------------+-----------+ Total placer CPU time: 12 secs Dumping design to file SevenSegClock_SevenSegClock.dir/5_1.ncd. 0 connections routed; 911 unrouted. Starting router resource preassignment WARNING - par: The driver of primary clock net clk_c is not placed on one of the sites dedicated for primary clocks. This primary clock will be routed to an H-spine through general routing resource and might suffer from excessive delay or skew. WARNING - par: The driver of primary clock net latch_c is not placed on one of the sites dedicated for primary clocks. This primary clock will be routed to an H-spine through general routing resource and might suffer from excessive delay or skew. Completed router resource preassignment. Real time: 19 secs Start NBR router at 23:29:01 03/10/17 ***************************************************************** Info: NBR allows conflicts(one node used by more than one signal) in the earlier iterations. In each iteration, it tries to solve the conflicts while keeping the critical connections routed as short as possible. The routing process is said to be completed when no conflicts exist and all connections are routed. Note: NBR uses a different method to calculate timing slacks. The worst slack and total negative slack may not be the same as that in TRCE report. You should always run TRCE to verify your design. ***************************************************************** Start NBR special constraint process at 23:29:01 03/10/17 Start NBR section for initial routing at 23:29:01 03/10/17 Level 4, iteration 1 20(0.02%) conflicts; 0(0.00%) untouched conn; 0 (nbr) score; Estimated worst slack/total negative slack<setup>: 278.832ns/0.000ns; real time: 20 secs Info: Initial congestion level at 75% usage is 0 Info: Initial congestion area at 75% usage is 0 (0.00%) Start NBR section for normal routing at 23:29:01 03/10/17 Level 4, iteration 1 17(0.02%) conflicts; 0(0.00%) untouched conn; 0 (nbr) score; Estimated worst slack/total negative slack<setup>: 278.832ns/0.000ns; real time: 20 secs Level 4, iteration 2 4(0.00%) conflicts; 0(0.00%) untouched conn; 0 (nbr) score; Estimated worst slack/total negative slack<setup>: 277.633ns/0.000ns; real time: 20 secs Level 4, iteration 3 0(0.00%) conflict; 0(0.00%) untouched conn; 0 (nbr) score; Estimated worst slack/total negative slack<setup>: 277.633ns/0.000ns; real time: 20 secs Start NBR section for setup/hold timing optimization with effort level 3 at 23:29:01 03/10/17 Start NBR section for re-routing at 23:29:02 03/10/17 Level 4, iteration 1 0(0.00%) conflict; 0(0.00%) untouched conn; 0 (nbr) score; Estimated worst slack/total negative slack<setup>: 277.633ns/0.000ns; real time: 21 secs Start NBR section for post-routing at 23:29:02 03/10/17 End NBR router with 0 unrouted connection NBR Summary ----------- Number of unrouted connections : 0 (0.00%) Number of connections with timing violations : 0 (0.00%) Estimated worst slack<setup> : 277.633ns Timing score<setup> : 0 ----------- Notes: The timing info is calculated for SETUP only and all PAR_ADJs are ignored. Total CPU time 14 secs Total REAL time: 21 secs Completely routed. End of route. 911 routed (100.00%); 0 unrouted. Hold time timing score: 0, hold timing errors: 0 Timing score: 0 Dumping design to file SevenSegClock_SevenSegClock.dir/5_1.ncd. All signals are completely routed. PAR_SUMMARY::Run status = completed PAR_SUMMARY::Number of unrouted conns = 0 PAR_SUMMARY::Worst slack<setup/<ns>> = 277.633 PAR_SUMMARY::Timing score<setup/<ns>> = 0.000 PAR_SUMMARY::Worst slack<hold /<ns>> = 0.322 PAR_SUMMARY::Timing score<hold /<ns>> = 0.000 PAR_SUMMARY::Number of errors = 0 Total CPU time to completion: 14 secs Total REAL time to completion: 21 secs par done! Copyright (c) 1991-1994 by NeoCAD Inc. All rights reserved. Copyright (c) 1995 AT&T Corp. All rights reserved. Copyright (c) 1995-2001 Lucent Technologies Inc. All rights reserved. Copyright (c) 2001 Agere Systems All rights reserved. Copyright (c) 2002-2017 Lattice Semiconductor Corporation, All rights reserved.


Late 20s, no children. Worked great as far as birth control. Sklya insertion was extremely painful. I spotted on and off for about a month. My period decreased however my cramps increased times 10. It has been 5 months and I was in a lot of pain. I previously would only have cramps on the day my cycle started but now it was the entire week leading up to it.
I gave it till this long thinking my body would adjust.
SENT8
Just went to the doctor and she stated that it was still placed correctly but it was not as high up as it should be. I decided to have it removed. I felt an immediate relief! As if my body did not want that thing in there. Switching back to Nuva Ring.


The final leg of #BayaniChallenge 2016 held in Metro Manila was participated by thousands of volunteers from Globe, Singtel (Singapore), Optus (Australia), Convergys Alabang, 24/7 Makati, GK Mabuhay, GK employees, GK residents, youth leaders, and individual volunteers.
Fitness First, one of the world’s leading health and fitness companies, recently turned over 22 houses in Tapaz, Capiz to families affected by Typhoon Yolanda. Done in partnership with Gawad Kalinga, the Fitness First Gawad Kalinga Village signifies the company’s commitment to give back to the community in which they operate.
Are you up for the ultimate summer adventure this year? Have a meaningful and fulfilling one with Gawad Kalinga and other volunteers, and join this year’s Bayani Challenge.
As Filipinos celebrated National Heroes Day on Aug. 25, about 1,000 volunteers in Negros Occidental did their little acts of heroism by heading to four different sites to engage in Bayanihan activities.
Philippines, July 11, 2014 – Gawad Kalinga’s latest SIBOL Learning Center sponsored by global bank Credit Suisse, officially opened on June 25 at GK Santa Ana Village, Pateros, Metro Manila, benefitting 30 children aged from three to four years old. In addition, Credit Suisse is also supporting a SIBOL education program package for 30 children at the center for one year, covering teachers’ training, school tuition for the 30 students and procurement of school supplies.


Utility functions for checking function parameters and throwing appropriate errors.
public static function checkArgument(expression:Boolean, message:String = null):void
Check that the specified expression is true.
Parameters
| expression:Boolean |
| |
| message:String (default = null )
|
Throws
public static function checkIndex(index:int, size:int, message:String = null):int
Check that the specified index is valid: greater than or equal to 0, and less than size.
Parameters
| index:int |
| |
| size:int |
| |
| message:String (default = null )
|
Returns
| int — the index that was checked.
|
Throws
public static function checkNotNull(ref:*, message:String = null):*
Check that the reference is not null (or undefined) and return it as a convenience.
Parameters
| ref:* |
| |
| message:String (default = null )
|
Returns
| * — the reference that was checked.
|
Throws
public static function checkRange(value:Number, low:Number, high:Number, message:String = null):Number
Check that the specified value is not NaN and between the low and high values (inclusive).
Passing NaN for low or high will disable that test.
Parameters
| value:Number |
| |
| low:Number |
| |
| high:Number |
| |
| message:String (default = null )
|
Returns
| Number — the value that was checked.
|
Throws
Copyright © 2007-2009 Three Rings Design, Inc.


UFC News: Montana De La Rosa vs Mara Romero reportedly in the works for February 15th event
by Soumik DattaAccording to a report from MMA Junkie, a potential UFC Women's Flyweight bout between Montana De La Rosa and Mara Romero Borella is reportedly in the works for early 2020 as part of the UFC's February 15th card that the promotion is yet to confirm.
Montana De La Rosa and Mara Romero Borella's run in the UFC
Having made her UFC in 2017, Montana De La Rosa had finished off most of her opponents in the Octagon via submission, prior to the recent defeat that she suffered in June at the hands of Andrea Lee. The loss at UFC on ESPN+ 12 ended De La Rosa's four-fight winning streak, however, she has secured vital wins in the UFC against the likes of Cristina Marks, Rachael Ostovich and Nadia Kassem.
On the other hand, Mara Romero Borella also signed with the UFC in 2017 and in her first fight she defeated Kalindra Faria via submission but suffered a loss to Kaitlyn Chookagian. In February, Borella defeated Taila Santos via submission, however, she lost her last Octagon outing to Lauren Murphy in the third round via strikes.
Montana De La Rosa vs Mara Romero Borella set for UFC's February 15th event
As of now, an official announcement is yet to be made by the UFC in terms of a potential Flyweight bout between De La Rosa and Romero Borella, however, following a recent Instagram post from the former, it looks like the two women could be set for a showdown in the Octagon on the 15th of February.


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{%extends "base/page.html"%} {%block title%}Versioning policy{%endblock%} {%block contents%}
We always recommend that all users run the latest available minor release for whatever major version is in use.
PostgreSQL major releases include new features and occur roughly once every year. A major release is numbered by increasing either the first or second part of the version number, e.g. 9.1 to 9.2.
Major releases usually change the internal format of system tables and data files. These changes are often complex, so we do not maintain backward compatibility of all stored data. A dump/reload of the database or use of the pg_upgrade module is required for major upgrades.
Minor releases are numbered by increasing the third part of the version number, e.g. 9.2.3 to 9.2.4. The PostgreSQL team only adds bug fixes to minor releases. All users should upgrade to the most recent minor release as soon as possible. While upgrades always have some risk, PostgreSQL minor releases fix only frequently-encountered, security, and data corruption bugs to reduce the risk of upgrading. The community considers not upgrading to be riskier than upgrading.
Upgrading to a minor release does not require a dump and restore; merely stop the database server, install the updated binaries, and restart the server. For some releases, manual changes may be required to complete the upgrade, so always read the release notes before upgrading.
The PostgreSQL project aims to fully support a major release for five years.
After a release falls out of full support, we may (at our committers' discretion) continue to apply further critical fixes to the source code, on a best-effort basis. No formal releases or binary packages will be produced by the project, but the updated source code will be available from our source code control system.
This policy will be followed on a best-effort basis. In extreme cases it may not be possible to support a release for the planned lifetime; for example if a serious bug is found that cannot be resolved in a given major version without significant risk to the stability of the code or loss of application compatibility. In such cases, early retirement of a major version may be required.
| Version | Current minor | Supported | First release date | EOL date |
|---|---|---|---|---|
| {{v.tree}} | {{v.tree}}.{{v.latestminor}} | {{v.supported|yesno:"Yes,No"}} | {{v.firstreldate|date:"F Y"}} | {{v.eoldate|date:"F Y"}} |


| resolved {future} | R Documentation |
Check whether a future is resolved or not
resolved(x, ...)
x |
A Future, a list, or an environment (which also includes list environment. |
... |
Not used. |
This method needs to be implemented by the class that implement
the Future API. The implementation should return either TRUE or FALSE
and must never throw an error (except for FutureError:s which indicate
significant, often unrecoverable infrastructure problems).
It should also be possible to use the method for polling the
future until it is resolved (without having to wait infinitely long),
e.g. while (!resolved(future)) Sys.sleep(5)
.
A logical of the same length and dimensions as x
.
Each element is TRUE unless the corresponding element is a
non-resolved future in case it is FALSE.


my work experience and education
- i hold a diploma degree in spicial education
- i worked at 'al-namothajia school for spicial education' for 2 years
- i worked at 'al-masar school for children with spicial needs' for 2 years
- i worked at 'al-furqan kindergarten' for 2 years
top ten movies that i like
- eat, pray, love
- a good year
- fast and fuirous
- jamanji
- the maze
- catch me if you can
- the lord of the rings
- harry potter
- once upone the time
- the room


11/08/2021
👩🏫
General:Meeting with Dr. Jimenez to discuss our idea about quorum sensing and to hear about the projects in his lab.
11/08/2021
👩🏫
General:Meeting with Mark Isalan to discuss our idea about evolving a quorum sensing protein. He suggests starting with specific documentation of AHL molecules and the LuxR family proteins that bind them.
11/08/2021
👩🏫
General:Meeting with Prof. Guy Bart Stan to discuss our idea about quorum sensing from a mathematical perspective.
11/08/2021
👩🏫
General:Meeting with SynBIC to discuss the admin side of our project. They gave us advise on reaching out to sponsors, finding a primary supervisor, and how to divide the work between us whilst COVID restrictions remained in place.
11/08/2021
General:Happy Birthday to David! 🎂
Chose the track of our project: "Molecular Evolutionary Outcomes".
11/08/2021
👩🏫
General:Meeting with our PhD advisor Alexis Casas.
11/08/2021
General:Started researching structural info on EsaR/I, methods of generating non-cognate inducer, possible list of non-cognate AHLs.
11/08/2021
General:Found initial list of non-cognate inducers from SigMol, reviewed experimentally-reviewed structural data on EsaR and EsaI, reviewed literature on the key residues involved in AHL binding, reviewed methods for generating the mutation library
11/08/2021
General:More research on EsaR and potential non-cognate AHLs we could target. Filled out the iDEC RRI form
11/08/2021
General:Contacted organisations at Imperial for funding.
Dry Lab:Planned out the bioinformatics experiments for structure prediction of EsaR and docking of the predicted structure with AHL ligands.
11/08/2021
👩🏫
General:Meeting with our supervisor Mark Isalan. Made our Twitter account! Made a spreadsheet of life sciences companies we could contact to seek funding from. Completed the Responsible Research Information form.
Wet Lab:Decided on and planned out saturation mutagenesis experiments for generating our library of mutants
11/08/2021
General:Emailed Professor Mike Sternberg and the Computational Biology Society to seek advice on our bioinformatics workflow. Emailed a researcher who previously worked on EsaR to see if she would be willing to speak with us.
Dry Lab:Began structural prediction of EsaR using I-TASSER and JPred.
11/08/2021
General:Reviewed more literature on the AHL binding domain of quorum sensing proteins.
Wet Lab:Revised our experimental plans for using site saturation mutagenesis.
11/08/2021
Dry Lab:Submitted a structure prediction job on Robetta.
11/08/2021
👩🏫
Dry Lab:Meeting with Charlie from Computational Biology Society/MSc Bioinformatics who has kindly provided advice on structural prediction software.
Wet Lab:Reviewed possible methods for screening and selecting for library variants.
11/08/2021
General:Drafted a pitch document that outlines our project to send to organisations when asking for support. Emailed Faculties at Imperial to seek financial support or spare consumables.
11/08/2021
General:Wrote up and submitted our team abstract for iDEC.
11/08/2021
General:Created a pitch deck for sponsors, edited our pitch email for sponsors to be more business-oriented. Started a wiki timeline log. Created a team Benchling account which we used throughout the competition to design primers and plasmids, and keep track of sequences and protocols.
11/08/2021
General:Completed sponsorship tier, email and pitch deck documents.
11/08/2021
General:Set up a meeting for our team member David to show us how to use Github. Collated a massive spreadsheet, inherited from previous Imperial synbio teams, with a collection of contacts from many biotech companies - we will be going through the contact list systematically to seek financial support and sponsoring.
11/08/2021
General:Meeting for github intro. Laima (our resident Estonian) emailed Estonian organisations to ask for financial support. Attempted to reach out to other students by introducing ourselves and our project on SynbioUK Slack channel.
Wet Lab:Had a discussion on possible screening methods, particularly using replica plating.
11/08/2021
General:Split our giant spreadsheet of biotech contacts into 10 so that each team member would email about 20-30 people. For each email we included our pitch deck and a document outlining what kind of publicity we would provide for different levels of sponsorship.
Dry Lab:Completed structure prediction of EsaR using multiple software. Used PyMOL to visualise and compare results across software.
11/08/2021
General:The Imperial Life Sciences Department kindly agreed to provide some funding for us to spend on lab consumables.
Dry Lab:Analysed the predicted structures on PyMOL and compared them to the crystallised homolog TraR (PDB: 1H0M).
11/08/2021
General:Filled out the Bio1 form which is required for us to begin wet lab work in the Hackspace. Organized a Benchling tutorial led by team member Aarushi.
Dry Lab:Meet with Charlie (MSc Bioinformatics) to discuss our structure prediction results and to ask about next steps in docking.
Wet Lab:Placed orders for the non-cognate AHLs we would use as target molecules for our variant library.
11/08/2021
General:Finalised the design for a plasmid construct containing the EsaR gene and its promoter, and placed an order for it from Twist Biosciences. Attended an iDEC webinar.
Dry Lab:Worked with Charlie (MSc Bioinformatics) to use AlphaFold2 for structure prediction just one day after its code was made public through a Nature article (https://www.nature.com/articles/s41586-021-03819-2).
11/08/2021
Dry Lab:Using ProteinsPlus (https://proteins.plus/), analyzed protein-ligand interactions for the predicted EsaR structures and its cognate ligand 3OC6HSL.
11/08/2021
Dry Lab:Analysed protein-ligand interactions using PDBsum (http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl?pdbcode=index.html). Visualised protein-ligand interactions on PyMOL.
Wet Lab:Prepared 20 LB agar plates.
11/08/2021
Dry Lab:Manually analysed key residue interactions between the predicted EsaR structure and its cognate 3OC6HSL on PyMOL.
Wet Lab:Prepared more LB agar plates with chloramphenicol. Created AHL stock solutions.
11/08/2021
Dry Lab:Finalised 2D maps of key residues involved in the protein-ligand interaction on LigPlot and ProteinsPlus. Decided to proceed with LigPlot as it is more widely recommended in the literature. Identified the coordinates of the ligand binding pocket for docking.
11/08/2021
General:Happy Birthday to Isabelle! 🎂
Dry Lab:Docked the ligand to a predicted EsaR structure using DockThor.
11/08/2021
General:Had our biweekly meeting where the progress and next steps for dry and wet lab were discussed. Online meeting with Evonetix to discuss a sponsorship!
Dry Lab:Made some slides to showcase our progress in bioinformatics so far.
Wet Lab:Wet lab meeting to collate the list of reagents and go over the protocols and methods.
11/08/2021
Wet Lab:Created a detailed flowchart of wet lab tasks and experiments.
11/08/2021
General:Happy birthday to Lucy! 🎂
We had an in-person meeting with a potential sponsor. Online meeting with a company see whether we could borrow some of their lab equipment. Emailed lab technicians from the Department of Life Sciences at Imperial to ask whether any labs had some consumables they could spare us.
11/08/2021
👩🏫⚠💡
General:Contacted Tom Ellis to ask for advice.
Dry Lab:Finalised the identification of key residues from 2D maps.
Wet Lab:Made a bacterial starter culture for electrocompetent cells, but didn't realise the Hackspace had ran out of spectrophotometer cuvettes and we could not measure OD600, and we didn't realise that the centrifuge at the Hackspace had tripped a wire and broke, so we could not proceed with making electrocompetent cells.
Our reflections:Looking back on this two months later, we were severely inexperienced in the lab at this time and this is a great example that shows our lack of foresight during the earliest stages of our time in the lab. We've grown so much since then!
11/08/2021
⚠💡
Wet Lab:We asked around if anyone could lend us cuvettes as soon as possible (we emailed about 4 different groups, and they all said they could provide us some) while the Hackspace also placed an order for them.
Our reflections:We were pretty resourceful but we were not yet perceptive of what sorts of lab consumables were commonplace and how difficult it would be to get them. Cuvettes are common for most labs so there was no need for us to ask so many people, and we didn't know how quickly certain consumables could be delivered.
11/08/2021
Dry Lab:Identified key residues from two additional predicted structures after docking: on Phyre and AlphaFold.
11/08/2021
General:Organized a team social at Nando's!
Wet Lab:Successfully made electrocompetent cells!
11/08/2021
General:Started writing a mini literature review on quorum sensing transcription regulators.
11/08/2021
General:Posted a second note about our project in SynbioUK Slack channel. Had an online meeting with Twist Biosciences to seek advice on our library generation methods. Secured a number of free Sanger sequencing reactions from Genewiz!
11/08/2021
👩🏫💡
General:Had an in-person meeting with Mike Tennant from the Centre of Environmental Policy at Imperial who offered us advice on how to pitch our ideas. Emailed our primary supervisor Mark for feedback on our mutagenesis and screening protocol.
Wet Lab:Designed primers for our site-saturation mutagenesis protocol - each target residue would be mutated using one mutagenic primer, and to obtain a protein with 4 mutated primers we would carry out sequential rounds of mutagenic PCR.
Our reflections:This was one of the first methods of mutagenesis that we were planning to use. Throughout the rest of the month with input from multiple different advisors, we had pivoted to different methods several times since advisors often gave contradictory advice. At the time we were seeking for a single right method to carry out our experiment, whereas we have since learnt that there can be multiple correct ways to generate our library and that different people give different advice based on what's historically worked for them. We should trust our own scientific instincts more and understand that every scientist has their own preferred methods.
11/08/2021
Wet Lab:Further worked on our primer design. Discussed our site saturation mutagenesis method based on feedback from Mark and the representatives from Twist.
11/08/2021
General:Reviewed team's chart of deadlines and progress.
11/08/2021
Wet Lab:Ordered primers!
11/08/2021
Dry Lab:The dry lab team worked on a Python script that would generate the sequences for all 20 000 of our mutants - these sequences would be used for in silico analysis and screening.
11/08/2021
⚠💡
Dry Lab:Edited the AlphaFold script on Google Colab to allow the code to loop through multiple sequences, so that we would not have to manually submit each variant.
Wet Lab:Created stock solutions of our AHLs.
Our reflections:We were still quite inefficient in the lab and small tasks like creating stock solutions would take us relatively long amounts of time. We realise that efficiency in the lab comes a lot from confidence and experience.
11/08/2021
Dry Lab:Wrote up a draft for the Methods section of the report.
11/08/2021
Dry Lab:Finalised the Python script to generate mutated sequences.
11/08/2021
Dry Lab:Read more literature on various softwares used for in silico saturation mutagenesis and tried some of them out.
11/08/2021
⚠💡
General:We found out that the Hackspace would be closed for a week starting next week and scrambled to find other alternatives for lab space. We were worried that we would lose a week of wet lab work, which would be a significant hit given how limited our time was. We emailed our primary supervisor Mark, the lab technicians from the Department of Life Sciences, and companies that we thought could arrange lab space for us.
Dry Lab:Call with the dry lab team to discuss the dry lab workflow. Meeting with Charlie (MSc Bioinformatics) to discuss in silico methods for saturation mutagenesis - he advised us that it would be unrealsitic to screen through all 20 000 variants in silico.
Our reflections:We had set many unrealistically high goals for ourselves, such as screening through the structures of all 20 000 variants in silico - we were quite unwilling to admit to ourselves how little time we had left for the project, and we also did not have a good understanding of the capacity of existing methods.
11/08/2021
⚠💡
General:Our plasmid from Twist was going to be delayed, and all of our wet lab experiments had been planned around this plasmid. We discussed how we could adapt to this unfortunate news. We decided that we could perform all experiments on LuxR first since it is an EsaR homolog, so we could familiarise ourselves with the experiments and still collect useful data, then once the plasmid containing EsaR arrives we would be able to jump right in.
Dry Lab:Tested mCSM-lig as a software to analyse how a mutant protein may interact differently with a given ligand.
Our reflections:It felt like everything was going wrong this week - we only had one and a half months left and we suddenly had no lab space and no gene. We felt really disappointed in ourselves, but persisted. We were forced to think of solutions and be as resourceful as possible. Although this was not ideal, ultimately this experience made us much better scientists!
11/08/2021
👩🏫
General:Drafted the Introduction section of the report. Arranged with the Department of Life Sciences to borrow their gel tanks.
Dry Lab:Arranged a call with Dr Suhail A Islam from the Centre of Integrative Systems Biology and Bioinformatics. He provided us guidance on what we could do for dry lab in the remaining time we had.
Wet Lab:Made some stock solutions (e.g. 20% Glc).
11/08/2021
General:Arranged with the Department of Life Sciences to use teaching labs while the Hackspace was closed. Changed the delivery address for all pending deliveries to the South Kensington campus instead of the Hackspace (where the teaching labs were).
Dry Lab:Call with the dry lab team.
Wet Lab:Made some media and a starter culture for electroporation.
11/08/2021
General:Spent a productive last day in the Hackspace guessing if our team members were dog people or cat people.
11/08/2021
General:Had a tour in the Chain lab in South Kensington where we would be based while the Hackspace was closed.
Dry Lab:Succeeded in using our edited version of AlphaFold on Google Colab to loop through multiple sequences and predict their structures.
11/08/2021
General:Finalised the draft of our introduction.
Dry Lab:Attempted to find a way to automate the conversion of .pdb files (the output of AlphaFold) into .pdbqt files (the required input for AutoDock Vina)
11/08/2021
General:Started working in our primary supervisor Mark's lab, under the day-to-day supervision of Jure. When we were first organising lab space during the start of iDEC, COVID restrictions were still in place and it was unsure whether Mark's lab would have space to accommodate us.
Dry Lab:Attempted to use the SOAP of SwissDock as an alternate docking software to AutoDock Vina.
Wet Lab:Made up some primer stock solutions. Tested our mutagenesis method on GFP so see whether we could successfully introduce mutations using sequential rounds of mutagenic PCR, using the change in fluorescence as a visual confirmation.
11/08/2021
Wet Lab:Ran a KLD reaction to recircularise the GFP PCR product following gel extraction.
11/08/2021
⚠
General:Got an agreement from Genewiz to get their barcodes for free sequencing.
Dry Lab:Explore the use of FoldX to analyse stability of mutant structures.
Wet Lab:Running sequential rounds of PCR to introduce multiple mutations did not work. We hypothesize that the KLD components were interfering with PCR, so we tested methods of removing KLD in between PCR rounds.
11/08/2021
General:Set up a team Figma account to begin designing our iDEC wiki. Arranged with Jure to use the LuxR plasmids in his lab.
Dry Lab:Got AutoDock Vina to work to dock the ligand to mutated protein structures.
Wet Lab:Designed primers for site-saturation mutagenesis on the LuxR plasmids.
11/08/2021
👩🏫
General:Meeting with Prof Pietro Spanu to seek advice on cloning methods.
Dry Lab:Continued encountering problems with automated file conversion of .pdb to .pdbqt
Wet Lab:Completed another dry run of sequential mutagenic PCR on GFP. Transformed and plated the cells.
11/08/2021
Wet Lab:Completed a dry run of our screening and selection protocol on the cells containing the variant GFP library. Screening was performed using a 96-well plate on a microplate reader.
11/08/2021
⚠
General:Received confirmation from Twist that our plasmid was going to be further delayed. Decided that we could no longer wait for the plasmid and that we must find alternate ways to get the EsaR gene. We emailed labs that might have the gene, and brainstormed methods of gene assembly using oligos since the delivery time for oligos is much quicker than ordering an intact gene.
Dry Lab:Attempted to troubleshoot the various attempts at automating conversion of .pdb to .pdbqt
Wet Lab:Prepared interesting GFP variants for Miniprep and sequencing. Made a batch of electrocompetent cells for LuxR experiments. Analysed data that was collected yesterday by the microplate reader.
11/08/2021
💡
General:Discussed methods of gene assembly, particularly an end-to-end ligation method that Mark had previously developed for genes up to 1 kb in length. Began designing oligos for this end-to-end ligation method. Our insert (containing the EsaR gene and its promoter) would be around 1.4 kb long, and would require assembly of 13 oligos into the correct order in a single step reaction.
Our reflections:Assembling 13 oligos end-to-end in a single step reaction was an ambitious goal. Our PhD advisors thought it was highly unlikely to work. Our inexperience actually benefitted us in this moment - we were naive enough to pursue crazy ideas and take risks like this one. And luckily we had enough funding to buy these oligos.
11/08/2021
General:Happy birthday to Dana! 🎂
We drafted diagrams for the report and wiki and worked further on the wiki design. We also designed primers for Gibson assembly of EsaR - we were willing to try any and all possible methods of getting the gene at this point. In the mean time, we would continue working with LuxR.
Dry Lab:Tried using the Imperial bioinformatics server to run jobs.
11/08/2021
General:Discussed our different gene assembly methods and wrote up protocols for them.
11/08/2021
👩🏫
General:Discussed end point measurements and their optimization with our secondary PhD advisor Emily.
Wet Lab:Began screening WT-LuxR against non-cognate AHLs so that we could compare this data with the variant library later on. Loaded two microplates with a LuxR plasmid and two different AHLs.
11/08/2021
👩🏫
General:Discussed the future of our project with our day to day PhD supervisor Jure. Emailed the Flow Cytometry facility manager Jessica to set up a meeting.
Wet Lab:Began generating our LuxR variant library. We first used PCR to generate a mutagenic gene fragment and to amplify the plasmid backbone, then used Gibson assembly to put these two genes together.
11/08/2021
Wet Lab:Tested another round of Gibson assembly to generate our LuxR library, this time also adding a desalting step to see whether it would affect transformation efficiency. Transformed and plated cells with our LuxR variant library.
11/08/2021
General:Finished the design of the Home and About pages in Figma.
11/08/2021
👩🏫
General:Meeting with Jessica from the Flow Cytometry facility to discuss whether flow cytometry and cell sorting would be a suitable method for us to screen through our variant library. She gave us a demo of cell sorting and explained the process.
Wet Lab:We have a LuxR variant library! Picked out some colonies to send for sequencing. Replated a portion of the library on plates containing non-cognate AHLs.
11/08/2021
👩🏫
General:Carried out some lab chores: filled out pipette boxes and emptied out bins.
Dry Lab:Had a Teams meeting with Dr Islam to seek help with docking.
Wet Lab:Miniprepped colonies for sequencing. Discussed flow cytometry and questions we had about the method.
11/08/2021
General:Analysed the sequencing data. Planned in detail the different conditions we would test for assembling EsaR.
Dry Lab:Used BLAST to search for more homologues of EsaR and LuxR, and looked specifically in the Protein Data Bank for crystallised homologues. Manually annotated the interactions of homologues with their cognate ligands.
Wet Lab:Poured a big plate + some smaller ones with AHL and antibiotics. Did Gibson assembly. Made elextrocompetent cells and transformed them with the Gibson-assembled constructs. Prepared controls and samples for FACS.
11/08/2021
Dry Lab:Completed multiple sequence alignment of the homologous structures on Clustal Omega.
Wet Lab:Resuspended the cultures from yesterday in PBS, which is required for cell sorting. Brought our samples to the FACS facility and performed cell sorting! We selected cells that showed fluorescence against the non-cognate C8HSL. Then we let the 96 well-plates (containing the sorted cells) grow overnight.
11/08/2021
Dry Lab:Worked on writing up the dry lab results for the report.
Wet Lab:Used the microplate reader to measure data from the 96-well plates with cells sorted yesterday. Made our first attempt at end-to-end ligation assembly of EsaR, ran the products on a gel.
11/08/2021
General:Happy birthday to Laima! 🎂
We analysed microplate data.
11/08/2021
Wet Lab:Continued attempts to assemble EsaR. Transformed cells with our LuxR library for another round of FACS tomorrow.
11/08/2021
👩🏫
Dry Lab:Had another call with Dr Islam to discuss the outcome of our dry lab experiments and what we could do in the remaining days.
Wet Lab:Sent off samples to FACS. Grew some of our LuxR library on large culture dishes. Continued our assembly of EsaR and ran more gels to test conditions.
11/08/2021
Wet Lab:End of our days in the lab for the competition.


Hello, fellow developer!
This is the Para Web Console — a simple web interface which helps you easily manage your backend.
To get started, simply enter your credentials above and hit 'Connect'.
Don't have API keys? Get a free account from paraio.com.
If you ever get stuck, have a look at the docs
or the source.


| DOSE: |
|
insufflated |
Desoxypipradrol |
(powder / crystals) |
I just happened to have procured 2 grams of this material [2-DPMP) mainly just out of curiousity. I am a 22 year old male, very expierenced with drugs in general (stimulants like e, mdma, coke, amphetamine. Psychedelics lsd mushrooms morning glory. Dissociative ketamine and dxm and alot of pharmceuticals opiates, downers). Recently a friends and I have been purchasing RC and remembered about desoxypipradrol.

T0:00 it immediatly burned my nostril, I waited for the effects to start before I would finish the line. This is where the mistake was made, I was overly excited and after about 25-30 minutes I didnt feel any effect so I finished the line. 11 am would have been initial time of ingestion
T1:30 Feeling awake now but still weak. Music is improved and a definate mood lift. I call a few friends and also clean my room, definatly similar to methylphenidate or amphetamine.
T3:00 Mistakes were made with how potent this chemical really is. It take time to show itself but once it did I was jacked in hard. At the 3 hour point I was at my friends house, I had already redosed at my house and some more at his place while drinking a coffee, it hit me and I was jacked for 9 hours before any drop in intensity. During this time I mainly talked, played video games and smoked dope and did a few oxys because I realized I wasnt sleeping any other way
T3:00-12:00 This time can be described as being very focus on video games, I had not realize the amount of pressure on my jaw until my walk home at T13:00. I also had other plans that day but I completely forgot just jacked in the zone mario galaxy was my goal. seriously focused
T13:00- ??? I walked home very focused on moving quickly also ingested another 10mg oxycontin. I cant remember much about being on the walk even though its rather long one, I know I got home was wired for sound for a day and a half and even ate a 60 mg morphine the next day. Morphine seemed to innteract negetivly with 2dpmp, screwed with my breathing...avoid. oxycontin was fine
All in all I've used this substance a bunch of times much more responsibly. A few friends ate it and enjoyed it, expierencing mild side effects mostly prolonged wakefulness, sleep usually occuring with in 24 hours of ingestion. I've found very little problems dont take too late, dont do more than a dose, and this may allow you to see well ..... ghost
A few were very sensitive and were affected greatly, maybe an inability to metabolize the chemical? It induced hallucinations of seeing ghost, both friends were effected this way much like a deliriant. Both expierenced effects for a long period of time and lack of sleep definatly contributed to their expierence. I assumed it was just too much dopamine floating around and lack of sleep but 3 nights ago I felt a cat jump on my bed walk up by me put its nose close to my skin, felt a small cold area when this occured I opened my eyes to see the shadow like transparent cat named slash... my cat. a few months before a similar incident occoured in my basement but thats another story a sober story.
so I've used still expierencing no real overwhelming negitives and at night time I've expierenced some trippy things, more test in this field will occur.
All in all good like long length, high like amp or methylphenidate. my friends also ate multiple dose ages one ate like a bunch over 7 days and looped him self. so be smart. peace
[Reported Dose: '10-30mg']
-------
Follow-up Five Weeks Later:
I reported once on this substance and made mention of friends almost going psycho delirious .... datura like effect... one small proper dose is good 4 all day, three of my friends snorted and snorted and all them ended up with delirious effects. One went to the hospital on his own, which I've mention before.  In 3 or 4 days .... He saw people breaking in to his house, called the cops, once the cops seen him, they took him to the hospital. I've expierenced some problems as well due to its long half life, it almost accumulates in my body, well it does accumulate. Mild amphetamine-like psychosis but then I would take a break and be good.
[Reported Dose: '10-20......300mg in 4 days']
Experience Reports are the writings and opinions of the individual authors who submit them.
Some of the activities described are dangerous and/or illegal and none are recommended by Erowid.


--- layout: post title: ! 'Rails 1.1.4: Security fix without breakage' categories: - releases author: David published: true date: 2006-06-30 14:18:00.000000000 +01:00 ---
The security fix from Rails 1.1.3 might have closed the hole, but it also caused breakage for people with controllers in modules. We’ve fixed that now, so Rails 1.1.4 should work for any application that also ran under 1.1.2. We apologize for the problem with 1.1.3 and encourage everyone running 1.1.x to upgrade as soon as possible to this release.
Note: Edge Rails was never affected by this security issue as it includes a rewritten routes module. So if you’re running on the latest edge, you don’t need to worry about upgrading.


A flash of light streaks toward a creature of your choice within range. Make a ranged spell attack against the target. On a hit, the target takes 4d6 radiant damage, and the next attack roll made against this target before the end of your next turn has advantage, thanks to the mystical dim light glittering on the target until then.
At Higher Levels. When you cast this spell using a spell slot of 2nd level or higher, the damage increases by 1d6 for each slot level above 1st.


If the UNCALCED record is present in the Book stream, it indicates the Calculate message was in the status bar when Excel saved the file. This occurs if the sheet changed, the Manual calculation option was on, and the Recalculate Before Save option was off (Options dialog box, Calculation tab).
Record Data
|
Offset |
Name |
Size |
Contents |
|
4 |
(Reserved) |
2 |
Reserved; must be 0 (zero) |


Please enter the following information in order to process your concerns.
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{% include head.html %}
Graduated from the University of Liege (Master in Business Law - Cum Laude) with a deep interest in banking and financial law.
Admitted to the Luxembourg Bar on list II.
Advising on general investment fund and regulatory matters in relation to regulated and unregulated investment vehicles, including:
Assisting the ICM / Banking teams on Luxe,bourg law financing transactions.
Assisting the Investment Fund team on Luxembourg law matters
"De la délégation de la mission de conservation des actifs par le dépositaire sous la règlementation AIFM".
Mother tongue
C1, Excellent understanding, spoken and written English
B2, Good understanding, spoken and written German
B2, Good understanding and spoken Luxembourgish
A2, Able to understand a basic text, to introduce myself and ask for information
A1, Able to understand a basic text or a basic conversation
Awarded for stopping a nuclear meltdown, even though I also started it.
Honored by Duff Beer for being an outstanding customer several years straight. Qualifications included most beer consumed at a bar, most beer purchased, and most beer ralphed.
Specifically this prestigious awarded twice for stopping a robber with my belly.


You must declare the "declarativeNetRequest"
permission in the
extension manifest to use this API.
Host permissions are required if the extension
wants to redirect requests. You must also declare the
"declarative_net_request"
manifest key, which should be a
dictionary with a single key called "rule_resources"
. It should be
a list containing a single element, the path to a JSON file containing the
declarative rules to use, as shown below.
{ "name": "My extension", ... "declarative_net_request" : { "rule_resources" : ["rules.json"] }, "permissions": [ "declarativeNetRequest", "*://example.com/*" ], ... }
The JSON file specified as part of "rule_resources"
in the manifest
contains a list of rules. The number of rules that an extension can specify is
bound by the
chrome.declarativeNetRequest.MAX_NUMBER_OF_RULES constant.
A single declarative rule consists of four fields: id
,
priority
, condition
and action
. See the
Rule section below for more information on the format.
There are the following kinds of rules:
An example rule:
{ "id" : 1, "action" : { "type" : "block" }, "condition" : { "urlFilter" : "abc", "domains" : ["foo.com"], "resourceTypes" : ["script"] } }
The above rule will block all script requests originating from
"foo.com"
to any URL with "abc"
as a substring.
The urlFilter
field of a rule condition is used to specify the
pattern which is matched against the request URL. Some examples of URL filters:
urlFilter |
Matches | Does not match |
|---|---|---|
"abc" |
https://abcd.com https://example.com/abcd |
http://ab.com |
"abc*d" |
https://abcd.com https://example.com/abcxyzd |
http://abc.com |
"||a.example.com" |
https://a.example.com/ https://b.a.example.com/xyz |
http://example.com/ |
"|https*" |
https://example.com |
http://example.com/ http://https.com |
"example*^123|" |
https://example.com/123 http://abc.com/example?123 |
https://example.com/1234 https://abc.com/example0123 |
To disable the extension ruleset on a particular page, extensions can use the chrome.declarativeNetRequest.addAllowedPages API method. This allows extensions to specify match patterns that are compared against the tab URL. Any requests originating from these pages are not evaluated against the extension ruleset. The number of such patterns an extension can add is bound by the chrome.declarativeNetRequest.MAX_NUMBER_OF_ALLOWED_PAGES constant.
To add rules dynamically, extensions can use the chrome.declarativeNetRequest.addDynamicRules API method.
removeHeaders
rules are evaluated[3]. Rules from all extensions are evaluated
to determine the set of removed headers.
manifest.json
{ "name" : "declarativeNetRequest extension", "version" : "1", "declarative_net_request" : { "rule_resources" : ["rules.json"] }, "permissions" : ["*://google.com/*", "declarativeNetRequest"], "manifest_version" : 2 }
rules.json
[ { "id" : 1, "action": { "type" : "block" }, "condition" : {"urlFilter" : "google.com", "resourceTypes" : ["main_frame"] } }, { "id" : 2, "action" : { "type" : "allow" }, "condition" : { "urlFilter" : "google.com/123", "resourceTypes" : ["main_frame"] } }, { "id" : 3, "priority" : 1, "action" : { "type" : "redirect", "redirectUrl" : "https://example.com" }, "condition" : { "urlFilter" : "google.com", "resourceTypes" : ["main_frame"] } }, { "id" : 4, "priority" : 1, "action" : { "type" : "redirect", "redirectUrl" : "/a.jpg" }, "condition" : { "urlFilter" : "abcd.com", "resourceTypes" : ["main_frame"] } } ]
"http://google.com"
. Rules with id (1)
and (3) match. The request will be blocked because blocking rules have
higher priority than redirect rules.
"http://google.com/1234"
. Rules with
id (1), (2), and (3) match. Because the request has a matching
allow
rule, the request is not blocked nor redirected and
continues to "http://google.com/1234"
.
"http://abcd.com"
. The rule with id
(4) matches. Since rule (4) specifies a relative URL, the request is
redirected to "chrome-extension://<extension-id>/a.jpg"
.


Home
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Contact
Judy Zheng
collection. things
statics - still life. for more information on commissioned pieces please email.
@2021 by Judy Zheng


COMING SOON -- The king has gone missing, and in his place the court wizard rules. But the wizard's laws seem cruel and harsh, and every day more unfair rules are forced onto the people. The kingdom is coming to a breaking point. Will the king be found, or will the wizard rule on?


[We get a lot of questions by email, and we do our best to respond to each and every one personally. Though we have a short FAQ on the Rolling Jubilee website, many questions deserve more in-depth responses. We’ve decided to post longer answers to some of the more common questions on this blog, in a regular feature titled ‘Commonly asked questions’. Check back regularly for updates!]
“How do you choose whose debt to buy? / Why can’t you buy my debt?”
Some form of this is far and away our most frequently asked question. It’s often heartbreaking to read the stories of despair and frustration that accompany these questions. These stories overwhelmingly affirm the suspicion that crushing debt is very rarely the “fault” of the debtor – most often, their situation is precipitated by unfortunate circumstances far beyond their control, circumstances which could befall any of us.
So why can’t we just pick the “most” awful stories, the most “deserving” victims of the debt system, and buy their debt? The answer is twofold:
First, as far as we know, this is impossible. The way the debt buying process works is that essentially anonymous accounts are bundled together into large portfolios and sold as a whole. Before we buy the debt we don’t know whose debt we are purchasing. In one sense, this is a good thing. Imagine if debtors information (names, addresses, phone numbers, SSNs, …) was made available without having to buy the portfolio. This would be a serious breach of privacy. But the reason this is done is not to protect your privacy, but something far more banal: debt collectors – and the firms who sell to them – don’t care who you are. They only care that they’re making a good investment – that, as a whole, the package they’re buying will return more than they paid for it, even though any individual account might not. So debt sellers put together accounts from many sources and wrap them all together, so that debt collectors think they’re buying a package that will have enough accounts worth collecting on.
As a result, there is no way to enroll or sign up for the Jubilee.
In effect, the Rolling Jubilee is a somewhat random act of mutual
aid. And that gets to the second reason why we won’t just
pick and choose who to buy, even if we could. The Jubilee is meant
to help individuals, absolutely, but first and foremost it is
designed to be a campaign to educate the public about a predatory
system that puts people into debt for basic needs such as food,
housing, and education. In our view, none of these debts
are valid – they accumulate immorally large amounts of fees
and interest, and are the result of jacked-up original prices that
should never have existed in the first place, were it not for
out-of-control debt financing that inflated them to astronomical
levels.
Moreover, there is simply no way for us to buy all of the debt. The size of the secondary debt market is enormous – estimates vary, but seem to center in the high tens of billions, possibly over a hundred billion dollars per year. We’ve raised about $560,000 as of March 14th, 2013, enough to buy about 12 million dollars of debt. So it’s not simply a matter of getting more attention – we’d need to grow about a hundred thousand times larger to even handle a year’s worth of debt. As we’ve always said, Rolling Jubilee is “a spark, not the solution.” What we have to do, as a society, is start moving towards a world where healthcare, education, and other basic needs are not simply profit centers, not line items on a corporate balance sheet. Creating that world is the main mission of Strike Debt.
So, what do we tell people who are in debt right now,
and write to us asking that we buy their debt? We are
unable to give financial or legal advice, but that said, we can
point you toward people who may be able to help. If you live in New
York City and meet certain income restrictions, you can contact the
organization NEDAP,
(212) 925-4929, between 12 and 2 PM on Tuesdays-Thursdays. Or you can visit
CLARO:
http://www.brooklynvlp.org/


On or around 9:30 PM March 10, 2006, I consumed 5 mg of Amitriptyline in a cup of tea with approximately 0.250 gram of Chinese green tea (less than 30 mg caffeine). The amitriptyline-laced tea made my tongue feel numb. That numb feeling on my tongue from the amitriptyline-laced tea is similar to the numbness encountered with ibuprofen. This is also causally associated with the nausea experienced after taking amitriptyline. Thus the numbness on my tongue, the drug's pain relief and subsequent nausea indicate to me that, like ibuprofen, amitriptyline also suppresses pain. This is probably a cholinergic reaction to the medication.
Within 30 minutes of ingestion, I felt tired and went to sleep. On waking I experienced light nausea, anxiety and other symptoms associated with amitriptyline. Ataxia (lack of coordination in walking) was also present throughout the day with anxiety and daydreams. I feel that this medication would worsen my depression, since the anxiety indicates a hypoglycemic reaction and the daydreams indicate probably suppression of REM and its associated dream state.
Amitriptyline's pain-relieving side effect is associated with both the numbness on my tongue and the brief waves of nausea. All I have experienced so far are daydreams, which may be a mild form of hallucinations. I also didn't recognize my friend's voice on the phone, and my social skills are temporarily absent. After four hours awake, I'd recovered from most of the side effects of amitriptyline, except for occasional daydreams.
The negative side effects (grogginess and brief nausea on waking, followed by 6 hours of anxiety and 12 hours of daydreams) tend to outweigh the positive side effects (pain relief and sedation. For the length of sedation I experienced (7 hours of fitful sleep), the 12 hours of daydreams, 6 hours of anxiety, and the waves of grogginess (including ataxia) does not make amitriptyline a great sleeping aid.
Overall I feel that in cases of persons with a history of head trauma, borderline personality disorder, dysthymia (depression), anxiety and insomnia associated with hypoglycemia, amitriptyline only worsens the symptoms of depression and anxiety. However, it is a great sedative.
Timeline of my experience:
Physical condition: history of mild traumatic brain injury.
Rating of psychosocial condition: 4.5 out of 10. 10 = perfect
36 hours awake, feeling manic, had a busy day.
Day 1:
9:30 PM - ingested 5 milligrams of amitriptyline.
11:30 PM - retired to bed after spending an hour on the Internet.
Day 2:
6:30 AM - awoke; stayed in bed.
9:00 AM - had anxiety attack in shower due to the shower door coming off the rail holding it in place. Usually I can put the door back on without incident.
9:30 AM - 1:30 PM: anxiety and daydreams come and go in waves.
1:30 PM - anxiety seems to subside. Daydreams come and go for the next 8 hours
9:30 PM - no anxiety, and daydreams have subsided.
1:30 AM - retire to bed; fell asleep within one hour.
Rating of psychosocial condition: 3 out of 10
Day 3:
8:30 AM - awoke; took shower; had breakfast.
7:30 PM - Rating of psychosocial condition: 4 out of 10
Experience Reports are the writings and opinions of the individual authors who submit them.
Some of the activities described are dangerous and/or illegal and none are recommended by Erowid.


Portfolio of Quentin Ross
I, Quentin Ross, am a 3rd year student at the University of Michigan - Ann Arbor College of Engineeing, working towords a Bachelor's degree in Computer Science.
GitHub
Click here to read about my project experience.
Click here to check out my hobbies and personal interests.
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The best dash cam captures a top Black Friday bundle deal
by John McCannThe best dash cam captures a top Black Friday bundle deal
Get two dash cams, a memory card and a carry case
(Image credit: TechRadar)
The Nextbase 522GW is our best dash cam in the world right now, and it's just got even better thanks to this excellent Black Friday bundle deal from Very.
With the ability to capture Full HD video footage at 60fps, or QHD quality video at 30fps, you'll be safe in the knowledge that the Nextbase 522GW is capturing events with crystal-clear video.
The 3-inch HD touchscreen display allows for easy navigation of the interface, and with a 140 degree field of view, this front-facing camera can see what's happening in the surrounding lanes, as well as in yours.
- There are plenty of Black Friday deals available right now
- And we'll see more deals arrive on Cyber Monday 2019
- The best cheap dash cam deals
You're getting more than just a dash cam
You also get a rear window camera, which can capture crucial footage if you are rear-ended.
That's not all though, as you also get a 32GB microSD card and a carry case thrown in to this bundle, saving you £60 in total.
Considering the 522GW is £149.99 on its own, you're basically paying just £10 for the second camera (RRP: £49), case and microSD card - which is a real steal.
TechRadar is scouring every retailer and rounding up all the top deals over the Black Friday period, and we’ve put all the best Black Friday deals and Cyber Monday deals in easy-to-navigate articles to help you find the bargains you’re looking for.


public interface TelegramBotApiService
| Modifier and Type | Method and Description |
|---|---|
Single<ApiResponse<Boolean>> |
answerCallbackQuery(CallbackQueryAnswer callbackQueryAnswer)
Answer callback query single.
|
Single<ApiResponse<Boolean>> |
answerInlineQuery(InlineQueryAnswer inlineQueryAnswer)
Answer inline query single.
|
Single<ApiResponse<Boolean>> |
deleteMessage(ChatIdMessageIdRequest chatIdMessageIdRequest)
Use this method to delete a message.
|
Single<ApiResponse<Object>> |
editMessageCaption()
Edit message caption single.
|
Single<ApiResponse<Object>> |
editMessageReplyMarkup()
Edit message reply markup single.
|
Single<ApiResponse<Object>> |
editMessageText(MessageRequest messageRequest)
Use this method to edit text and game messages sent by the bot or via the bot (for inline bots).
|
Single<ApiResponse<Message>> |
forwardMessage(ForwardRequest forwardRequest)
Use this method to forward messages of any kind.
|
Single<ApiResponse<Chat>> |
getChat(ChatIdRequest chatIdRequest)
Use this method to get up to date information about the chat (current name of the user for one-on-one conversations, current username of a user, group or channel, etc.).
|
Single<ApiResponse<List<ChatMember>>> |
getChatAdministrators(ChatIdRequest chatIdRequest)
Use this method to get a list of administrators in a chat.
|
Single<ApiResponse<ChatMember>> |
getChatMember(ChatIdUserIdRequest chatIdUserIdRequest)
Use this method to get information about a member of a chat.
|
Single<ApiResponse<Long>> |
getChatMembersCount(ChatIdRequest chatIdRequest)
Use this method to get the number of members in a chat.
|
Single<ApiResponse<User>> |
getMe()
A simple method for testing the bot's auth token.
|
Single<ApiResponse<UserProfilePhotos>> |
getUserProfilePhotos(UserProfilePhotosRequest userProfilePhotosRequest)
Use this method to get a list of profile pictures for a user.
|
Single<ApiResponse<Boolean>> |
kickChatMember(ChatIdUserIdRequest chatIdUserIdRequest)
Use this method to kick a user from a group or a supergroup.
|
Single<ApiResponse<Boolean>> |
leaveChat(ChatIdRequest chatIdRequest)
Leave chat single.
|
Single<ApiResponse<Message>> |
sendAudio()
Send audio single.
|
Single<ApiResponse<Message>> |
sendChatAction()
Send chat action single.
|
Single<ApiResponse<Message>> |
sendContact()
Send contact single.
|
Single<ApiResponse<Message>> |
sendDocument()
Send document single.
|
Single<ApiResponse<Message>> |
sendLocation()
Send location single.
|
Single<ApiResponse<Message>> |
sendMessage(MessageRequest messageRequest)
Use this method to send text messages.
|
Single<ApiResponse<Message>> |
sendPhoto()
Send photo single.
|
Single<ApiResponse<Message>> |
sendSticker()
Send sticker single.
|
Single<ApiResponse<Message>> |
sendVenue()
Send venue single.
|
Single<ApiResponse<Message>> |
sendVideo()
Send video single.
|
Single<ApiResponse<Message>> |
sendVideoNote()
Send video note single.
|
Single<ApiResponse<Message>> |
sendVoice()
Send voice single.
|
Single<ApiResponse<Boolean>> |
unbanChatMember(ChatIdUserIdRequest chatIdUserIdRequest)
Use this method to unban a previously kicked user in a supergroup or channel.
|
@POST(value="getMe") Single<ApiResponse<User>> getMe()
User
object.Single
Observable which will emit an ApiResponse
object which wraps a User
object@POST(value="sendChatAction") Single<ApiResponse<Message>> sendChatAction()
@POST(value="kickChatMember") Single<ApiResponse<Boolean>> kickChatMember(@Body ChatIdUserIdRequest chatIdUserIdRequest)
Single
Observable which will emit an ApiResponse
object which wraps a Boolean
chatIdUserIdRequest
- a ChatIdUserIdRequest
object, built with the ChatIdUserIdRequest.Builder#create()
methodSingle
Observable which will emit an ApiResponse
object which wraps a Boolean
@POST(value="unbanChatMember") Single<ApiResponse<Boolean>> unbanChatMember(@Body ChatIdUserIdRequest chatIdUserIdRequest)
Single
Observable which will emit an ApiResponse
object which wraps a Boolean
chatIdUserIdRequest
- a ChatIdUserIdRequest
object, built with the ChatIdUserIdRequest.Builder#create()
methodSingle
Observable which will emit an ApiResponse
object which wraps a Boolean
@POST(value="deleteMessage") Single<ApiResponse<Boolean>> deleteMessage(@Body ChatIdMessageIdRequest chatIdMessageIdRequest)
Single
Observable which will emit an ApiResponse
object which wraps a Boolean
chatIdMessageIdRequest
- a ChatIdMessageIdRequest
object, built with the ChatIdMessageIdRequest.Builder#create()
methodSingle
Observable which will emit an ApiResponse
object which wraps a Boolean
@POST(value="editMessageText") Single<ApiResponse<Object>> editMessageText(@Body MessageRequest messageRequest)
messageRequest
- a MessageRequest
objectSingle
Observable which will emit an ApiResponse
object which wraps a Object
@POST(value="editMessageCaption") Single<ApiResponse<Object>> editMessageCaption()
@POST(value="editMessageReplyMarkup") Single<ApiResponse<Object>> editMessageReplyMarkup()
@POST(value="getChat") Single<ApiResponse<Chat>> getChat(@Body ChatIdRequest chatIdRequest)
Chat
object on success.chatIdRequest
- a ChatIdRequest
objectSingle
Observable which will emit an ApiResponse
object which wraps a Chat
object@POST(value="getChatAdministrators") Single<ApiResponse<List<ChatMember>>> getChatAdministrators(@Body ChatIdRequest chatIdRequest)
chatIdRequest
- a ChatIdRequest
objectSingle
Observable which will emit an ApiResponse
object which wraps a List
containing ChatMember
objects.@POST(value="getChatMembersCount") Single<ApiResponse<Long>> getChatMembersCount(@Body ChatIdRequest chatIdRequest)
Long
object on success.chatIdRequest
- a ChatIdRequest
objectSingle
Observable which will emit an ApiResponse
object which wraps a Long
object@POST(value="getChatMember") Single<ApiResponse<ChatMember>> getChatMember(@Body ChatIdUserIdRequest chatIdUserIdRequest)
ChatMember
object on success.chatIdUserIdRequest
- a ChatIdUserIdRequest
object, built with the ChatIdUserIdRequest.Builder#create()
methodSingle
Observable which will emit an ApiResponse
object which wraps a ChatMember
object@POST(value="getUserProfilePhotos") Single<ApiResponse<UserProfilePhotos>> getUserProfilePhotos(@Body UserProfilePhotosRequest userProfilePhotosRequest)
userProfilePhotosRequest
- a UserProfilePhotosRequest
object generated with UserProfilePhotosRequest.Builder#create()
Single
Observable which will emit an ApiResponse
containing a UserProfilePhotos
object@POST(value="sendMessage") Single<ApiResponse<Message>> sendMessage(@Body MessageRequest messageRequest)
Message
is returned.
For formatting options see: https://core.telegram.org/bots/api#formatting-options
messageRequest
- a MessageRequest
objectSingle
Observable which will emit an ApiResponse
object which wraps the sent Message
object@POST(value="forwardMessage") Single<ApiResponse<Message>> forwardMessage(@Body ForwardRequest forwardRequest)
forwardRequest
- a ForwardRequest
objectSingle
Observable which will emit an ApiResponse
object which wraps the sent Message
object@POST(value="sendPhoto") Single<ApiResponse<Message>> sendPhoto()
@POST(value="sendAudio") Single<ApiResponse<Message>> sendAudio()
@POST(value="sendDocument") Single<ApiResponse<Message>> sendDocument()
@POST(value="sendSticker") Single<ApiResponse<Message>> sendSticker()
@POST(value="sendVideo") Single<ApiResponse<Message>> sendVideo()
@POST(value="sendVoice") Single<ApiResponse<Message>> sendVoice()
@POST(value="sendVideoNote") Single<ApiResponse<Message>> sendVideoNote()
@POST(value="sendLocation") Single<ApiResponse<Message>> sendLocation()
@POST(value="sendVenue") Single<ApiResponse<Message>> sendVenue()
@POST(value="sendContact") Single<ApiResponse<Message>> sendContact()
@POST(value="leaveChat") Single<ApiResponse<Boolean>> leaveChat(@Body ChatIdRequest chatIdRequest)
chatIdRequest
- the chat id request@POST(value="answerCallbackQuery") Single<ApiResponse<Boolean>> answerCallbackQuery(@Body CallbackQueryAnswer callbackQueryAnswer)
callbackQueryAnswer
- the callback query answer@POST(value="answerInlineQuery") Single<ApiResponse<Boolean>> answerInlineQuery(@Body InlineQueryAnswer inlineQueryAnswer)
inlineQueryAnswer
- the inline query answer


É sempre importante colocarmos todos os dados requeridos, mas os dados obrigatórios para o cadastro são: login, senha, nome. Os demais poderão ser cadastrados posteriormente.
A dica senha é essencial para a recuperação da senha, não é obrigatório no momento do cadastro mas é de extrema importância para as próximas utilizações do aplicativo.
Kif, I have mated with a woman. Inform the men. This is the worst part. The calm before the battle. Bender, being God isn't easy. If you do too much, people get dependent on you, and if you do nothing, they lose hope. You have to use a light touch. Like a safecracker, or a pickpocket.
Example: There's no part of that sentence I didn't like! You, a bobsleder!? That I'd like to see!
Your lyrics lack subtlety. You can't just have your characters announce how they feel. That makes me feel angry! It's okay, Bender. I like cooking too. Interesting. No, wait, the other thing: tedious.
Example: Of all the friends I've had… you're the first. But I know you in the future. I cleaned your poop. Then we'll go with that data file!
Oh, I think we should just stay friends.  Aww, it's true. I've been hiding it for so long. Say it in Russian! Then throw her in the laundry room, which will hereafter be referred to as "the brig".
Example: We're rescuing ya. Robot 1-X, save my friends! And Zoidberg! Then we'll go with that data file! Okay, I like a challenge.
And I'm his friend Jesus. Oh right. I forgot about the battle.  We'll need to have a look inside you with this camera.
Example: Isn't it true that you have been paid for your testimony? Quite possible.
Não, a aba de matérias, funciona com o intuito de informar e descrever cada uma, as perguntas abaixo de sua descrição servem para o momento de conversação com o ChatBot.
Não, apenas uma opção pode ser escolhida.
Sentimos muito pelo transtorno, mas estamos aqui para melhorar e surpreende-lo. Dessa forma, contate-nos e envie uma sugestão, explicando o ocorrido!


| 021:072 Khan | : |
And We bestowed upon him Ishaque (Isaac), and (a grandson) Ya'qub (Jacob). Each one We made righteous. |
| 021:072 Maulana | : |
And We gave them Isaac; and Jacob, a son's son. And We made (them) all good. |
| 021:072 Pickthal | : |
And We bestowed upon him Isaac, and Jacob as a grandson. Each of them We made righteous. |
| 021:072 Rashad | : |
And we granted him Isaac and Jacob as a gift, and we made them both righteous. |
| 021:072 Sarwar | : |
We granted him Isaac and Jacob as a gift and helped both of them to become righteous people. |
| 021:072 Shakir | : |
And We gave him Ishaq and Yaqoub, a son's son, and We made (them) all good. |
| 021:072 Sherali | : |
And WE bestowed upon him Isaac, and a grandson, Jacob, and WE made all of them righteous. |
| 021:072 Yusufali | : |
And We bestowed on him Isaac and, as an additional gift, (a grandson), Jacob, and We made righteous men of every one (of them). |
Concepts/Themes Covered:
This tool uses eight English parallel translations for the Holy Quran imported from Mr. Clay Smith. You can also visit Qurany Concepts for browsing the Quran themes or topics. If you want to search the Holy Quran for keywords, please visit Qurany Keywords.


[gecode-users] IntVar::IntVar: Attempt to create variable with empty domain.
Jose A Magaña
jose_a_magana at hotmail.com
Tue Dec 24 19:43:35 CET 2013
Hello,
I am using Gecode to solve Conway Game of Life. It is an assignment for
the CSP course I am following at University.
My code is working correctly without any problems except for the trivial
case.
The objective is for a given initial solution add checkers to a board
until the configuration is stable (according to some neighbourhood rules).
The point is that when the initial solution is already stable I get an
exception: IntVar::IntVar: Attempt to create variable with empty domain.
The problem uses IntVar in the domain {0,1} and basically formalizes as
constraints relationships on the number of neighbours with certain
conditions depending on the 0/1 value of the cell.
I have found unsatisfiable cases where the same exception also occurs.
What kind of issues/conditions may cause this exception?
I am using version 4.2.1 in Windows.
Thanks in advance
Jose A. Magaña
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